Journal: Clinical and Experimental Immunology

Article Title: Cytotoxic T lymphocyte antigen 4-immunoglobulin G is a potent adjuvant for experimental allergen immunotherapy

doi: 10.1111/cei.12041

Figure Lengend Snippet: Experimental layout: In experiment 1, to evaluate that whether cytotoxic T lymphocyte antigen 4-immunoglobulin (CTLA-4–Ig) can enhance the suppressive effects of allergen-specific immunotherapy (SIT), it was combined with ovalbumin (OVA)-SIT (100 μg/mouse) in BALB/c mice. In experiment 2, CTLA-4–Ig is combined with OVA)-SIT in indoleamine 2,3 dioxygenase (IDO–/–) mice to examine whether the effect of CTLA-4–Ig is mediated by IDO. In experiment 3, to analyse the effects of CTLA-4–Ig on regulatory T cells the frequency of CD4+CD25+ forkhead box protein 3 (FoxP3)+ regulatory T cells (Treg) cells and Th2 cells were analysed 24 h after the last SIT injection and 24 h after inhalation challenges.

Article Snippet: Flow cytometry and antibodies Peridinin chlorophyll (Per-CP)-anti-CD4 (BD Pharmingen), fluorescein isothiocyanate (FITC)-anti-T1ST2 (also known as IL-33Ra) (MD-Biosciences, Zurich, Switzerland), phycoerythrin (PE)-anti-forkhead box protein 3 (FoxP3) and eFluor450-anti-CD25 (eBioscience, San Jose, CA, USA) were used for fluorescence activated cell sorting (FACS).

Techniques: Injection

Journal: Clinical and Experimental Immunology

Article Title: Cytotoxic T lymphocyte antigen 4-immunoglobulin G is a potent adjuvant for experimental allergen immunotherapy

doi: 10.1111/cei.12041

Figure Lengend Snippet: The effects of co-administration of cytotoxic T lymphocyte antigen 4-immunoglobulin (CTLA-4–Ig) with allergen-specific immunotherapy–ovalbumin (OVA-SIT) on the frequency of CD4+CD25+ forkhead box protein 3 (FoxP3)+ regulatory T cells (Treg) cells and T helper type 2 (Th2) cells in the blood. (a) Frequency of CD4+CD25+FoxP3+Treg cells in the blood 24 h after the last SIT injection; (b) frequency of CD4+CD25+FoxP3+Treg cells in the blood 24 h after the last inhalation challenge; (c) frequency of CD4+T1ST2+ [also known as interleukin (IL)-33Ra] T cells in the blood 24 h after the last SIT injection.

Article Snippet: Flow cytometry and antibodies Peridinin chlorophyll (Per-CP)-anti-CD4 (BD Pharmingen), fluorescein isothiocyanate (FITC)-anti-T1ST2 (also known as IL-33Ra) (MD-Biosciences, Zurich, Switzerland), phycoerythrin (PE)-anti-forkhead box protein 3 (FoxP3) and eFluor450-anti-CD25 (eBioscience, San Jose, CA, USA) were used for fluorescence activated cell sorting (FACS).

Techniques: Injection